Gene expression in human mesenchymal stem cell aging cultures: modulation by short peptides.

Ashapkin V, Khavinson V, Shilovsky G, Linkova N, Vanuyshin B. Mol Biol Rep. 2020.

Organ: bone-marrow · Type: in-vitro · Peptides: AED, KED, Vilon

In human embryonic bone-marrow MSCs aged by serial passage or by stationary culture, AED, KED, and KE at nanomolar concentrations modulated IGF1, FOXO1, TERT, TNKS2, and NFκB expression — culture work, not a therapy protocol.

Editorial summary of a 2020 original article in Molecular Biology Reports (DOI 10.1007/s11033-020-05506-3). Stem-cell aging cultures. Public pages cite PubMed only. Read the paper: PMID 32399807.

Methods

Human embryo bone-marrow mesenchymal stem cells (FetMSCs) aged in two models — serial “passages” or “stationary” culture — treated with short peptides Ala-Glu-Asp (AED), Lys-Glu-Asp (KED), and Lys-Glu (KE / Vilon). The authors measured expression of IGF1, FOXO1, TERT, TNKS2, and NFκB. Peptide concentrations are described as nanomolar; exact n belongs in the primary text.

Findings

The two aging models were similar in gene expression except TERT, which the authors report increased eightfold at stationary aging. IGF1 rose 3.5–5.6-fold with the peptides in both models. FOXO1 was about twice as high in stationary as in passage aging; KED inhibited FOXO1 1.6–2.3-fold; KE about doubled FOXO1 in the stationary model and did not affect it in the passage model. KED inhibited TNKS2 in the passage model and stimulated it in the stationary model. AED, KED, and KE stimulated NFκB in both models.

This is a single MSC-culture paper. It is not a bone-marrow therapy protocol. Follow the PMID.