Short Peptides Protect Fibroblast-Derived Induced Neurons from Age-Related Changes.

Kraskovskaya N, Linkova N, Sakhenberg E, Krieger D, Polyakova V, Medvedev D, Krasichkov A, Khotin M, Ryzhak G. Int J Mol Sci. 2024.

Organ: brain-cognition · Type: in-vitro · Peptides: EDR, KED, Epitalon

PMID: 39518916

In induced cortical neurons transdifferentiated from elderly-donor fibroblasts, EDR, KED, and AEDG increased dendritic arborization; EDR also lowered oxidative DNA damage — culture work, not a patient trial.

Editorial summary of a 2024 original article in International Journal of Molecular Sciences (MDPI, open access: doi:10.3390/ijms252111363). Public pages cite PubMed; the DOI is the OA record. Read the paper: PMID 39518916.

Methods

In-vitro model of human neuronal aging: transdifferentiation of aged dermal fibroblasts from elderly donors into induced cortical neurons. Cultures were treated with Glu-Asp-Arg (EDR / Pinealon), Lys-Glu-Asp (KED), or Ala-Glu-Asp-Gly (AEDG / Epitalon). Readouts included dendritic arborization, mitochondrial and lysosomal activity, p16, Lamin B1-related senescence marks as framed in the introduction, and oxidative DNA damage. Protocol numbers belong in the primary text.

Findings

The authors report that all three peptides promoted arborization of the dendritic tree, increasing both the number of primary processes and total dendrite length. The tripeptides had no effect on mitochondrial or lysosomal activity or on p16 protein in induced neurons. EDR reduced oxidative DNA damage in neurons derived from elderly-donor fibroblasts. They conclude that the peptides partially protect induced neurons from age-related changes and stimulate dendritogenesis in this system.

This is a single culture paper. Induced neurons are not patients. It is not a clinical trial and not a product claim. Follow the PMID and the OA DOI.