Pinealon increases cell viability by suppression of free radical levels and activating proliferative processes.

Khavinson V, Ribakova Y, Kulebiakin K, Vladychenskaya E, Kozina L, Arutjunyan A, Boldyrev A. Rejuvenation Res. 2011.

Organ: brain-cognition · Type: in-vitro · Peptides: Pinealon

PMID: 21978084

In cerebellar granule cells, neutrophils, and PC12 cells, Pinealon (EDR) restricted ROS accumulation and necrotic death in a dose-dependent way; higher concentrations also shifted ERK timing and the cell cycle.

Editorial summary of a 2011 original article in Rejuvenation Research (DOI 10.1089/rej.2011.1172). Cell-culture oxidative-stress work. Public pages cite PubMed only. Read the paper: PMID 21978084.

Methods

Synthetic tripeptide Pinealon (Glu-Asp-Arg) applied to cerebellar granule cells, neutrophils, and pheochromocytoma (PC12) cells under oxidative stress stimulated by receptor-dependent or receptor-independent processes. Readouts included reactive oxygen species (ROS), necrotic death by propidium iodide, ERK 1/2 activation timing, and cell-cycle modification. Concentrations and n belong in the primary text.

Findings

The authors report dose-dependent restriction of ROS accumulation in all three cell types, with decreased necrotic cell death. The protective effect was accompanied by delayed ERK 1/2 activation and modification of the cell cycle. ROS restriction and mortality reduction saturated at lower concentrations, while cell-cycle modulation continued at higher concentrations. They conclude that besides antioxidant activity, Pinealon may interact directly with the cell genome.

This is a single in-vitro communication. It is not a neuroprotection trial in patients. Follow the PMID.