Short Peptides Protect Oral Stem Cells from Ageing

Sinjari B, Diomede F, Khavinson V, Mironova E, Linkova N, Trofimova S, Trubiani O, Caputi S. Stem Cell Rev Rep. 2020.

Organ: general-aging · Type: in-vitro · Peptides: Epitalon, KED

In late-passage human periodontal-ligament and gingival mesenchymal stem cells, AEDG and KED lowered p16 and p21 mRNA and immunofluorescence relative to untreated passage-25 controls.

Editorial summary of a 2020 original article in Stem Cell Reviews and Reports. Culture work on oral mesenchymal stem cells. Public pages cite PubMed only. Read the paper: PMID 31677028.

Methods

Human periodontal ligament stem cells (hPLSCs) and human gingival mesenchymal stem cells (hGMSCs) at passage 25, split into untreated controls, AEDG (Epitalon), or KED. Senescence markers p16 and p21 were measured by RT-PCR and immunofluorescence. Fold changes versus control are those reported by the authors (AEDG 1.56–2.44-fold lower p16/p21 mRNA; KED 1.82–3.23-fold lower). Culture media, peptide concentrations, and image counts belong in the primary text.

Findings

The authors report that both peptides reduced p16 and p21 expression at late passage and that immunofluorescence matched the PCR direction. They suggest AEDG and KED as medium supplements to delay senescence-marker expression during large-scale expansion of oral hMSCs. They link the result to earlier geroprotective reports for these peptides in other cell and animal models.

This is a single in-vitro communication. It is not a stem-cell therapy protocol and not a product claim. Follow the PMID.